Nikon (De Rooij Group)

Nikon (De Rooij Group)

nikon_tirf_hubrecht_institute

Type
NIKON TIRF/Confocal microscope

Features:

  • “Live Cell” imaging
  • External emission and excitation filter wheels
  • Motorized XY-stage
  • Total Internal Reflection Fluorescence (TIRF) microscopy
  • Dual camera setup
  • Widefield fluorescence microscopy
  • Nikon C1 Confocal microscope
  • Perfect Focus System (PFS)

Temperature and CO2 control:

  • OkoLab Microscope Cage Incubator

Objectives:

  • 10x Dry N.A. 0.30 WD 15.2
  • 20x Dry N.A. 0.75 WD 1.0
  • 40x Dry N.A. 0.60 WD 3.6-2.8
  • 40x Oil N.A. 1.30 WD 0.2
  • 60x Oil N.A. 1.49 WD 0.12
  • 100x Oil N.A. 1.30 WD 0.20

TIRF Lasers:

  • 405 nm
  • 488/514nm
  • 594 nm

Confocal Lasers:

  • 405 nm
  • 488 nm
  • 594nm

External Excitation Filters:

  • CFP (430/24)
  • GFP (470/40)
  • YFP (500/20)
  • mCherry (572/35)

External Emission Filters:

  • CFP (470/40)
  • GFP (520/40)
  • YFP (535/30)
  • mCherry (640/50)