TIRF Nikon TE2000 – Roper FRAP system
Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Multi-position acquisition,
Electrical Stimulation, Perfusion Experiments, Phase Contrast Time Lapse Microscopy
Microscope: Nikon Eclipse TE2000 with Perfect Focus System
Objectives:
Apo TIRF 100x/1.49 oil, Plan Apo VC 100x/1.4 oil, Plan Fluor 40x/1.30 oil, Plan Apo 40x/1.0 oil,
Plan Apo 20x/0.75 dry
Beamsplitter: DV 2 (two-channel, simultaneous-imaging system)
Camera:
Photometrics Evolve 512 EMCCD
Photometrics CoolSNAP HQ2 CCD
Lasers:
Vortran 405 nm (100 mW)
Cobolt Calypso 491 nm (100 mW)
Cobolt Jive 561 nm (100 mW)
FRAP: ILas0 system (Roper Scientific France/Institut Curie)
Filters:
ET-DAPI (49000), ET-CFP (49001), ET-GFP (49002), ET-YFP (49003), ET-DSRed (49005), ETCy5
(49006), ET-mCherry (49008), ET-CFP/YFP (59017), ET-GFPmCherry (59022) – all Chroma
Stage: Prior
Filter Wheels: Sutter LB10-3 (excitation filter wheel with smart shutter; emission filter wheel; smart shutter for transmission light)
Heating/CO2: Tokai Hit INUG2E-ZILCS Stage Top Incubator
Software: MetaMorph 7.7
Other equipment:
Narishige NT-88-V3 micromanipulator, Master-8 (A.M.P.I.), High-current stimulus isolator (World
Precision Instruments), Pressurized flow system with computer-controlled valves (Harvard
Apparatus)
Contact:
Dr. Ilya Grigoriev
The head of the Biology Imaging Center
Division of Cell Biology, Department of Biology, Faculty of Science, Utrecht University
Room O-504
Kruytgebouw,
Padualaan 8, 3584 CH Utrecht,
The Netherlands
+31-30-2533297
I.S.Grigoriev@uu.nl
http://cellbiology.science.uu.nl/biology-imaging-center-bic