TIRF Nikon Ti – Roper iLas FRAP/Photoablation system

Andrea Marques and Stine Morthorst are studying the exocytosis of Rab6 vesicles

Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)

Microscope: Nikon Eclipse Ti with Perfect Focus System

Objective: Nikon Apo TIRF 100x N.A. 1.49 oil

Beamsplitter: Optosplit III (allows two or three color simultaneous imaging)

Camera:
Photometrics Evolve 512 EMCCD
Photometrics CoolSNAP HQ2 CCD

Lasers:
Vortran Stradus 405 nm (100 mW)
Cobolt Calypso 491 nm (100 mW)
Cobolt Jive 561 nm (100 mW)
Vortran Stradus 642 nm (110 mW)
Teem Photonics 532 nm Q-switched pulsed laser (for photoablation)

FRAP: ILas system (Roper Scientific France/ PICT-IBiSA, Institut Curie)

Filters:
ET-DAPI (49000), ET-CFP (49001), ET-GFP (49002), ET-YFP (49003), ET-DSRed (49005), ETCy5
(49006), ET-mCherry (49008), ET-CFP/YFP (59017), ET-GFPmCherry (59022) – all Chroma

Stage: ASI motorized stage MS-2000-XY System for Inverted Microscope Nikon Te/Ti 2000

Filter Wheels: Sutter LB10-3 (excitation filter wheel with smart shutter; emission filter wheel; smart shutter for transmission light)

Heating: Tokai Hit INUBG2E-ZILCS Stage Top Incubator

Software: MetaMorph 7.7

Resolution: 63 nm in 1 pixel

 

Contact:

Dr. Ilya Grigoriev
The head of the Biology Imaging Center
Division of Cell Biology, Department of Biology, Faculty of Science, Utrecht University

Room O-504
Kruytgebouw,
Padualaan 8, 3584 CH Utrecht,
The Netherlands
+31-30-2533297
I.S.Grigoriev@uu.nl
http://cellbiology.science.uu.nl/biology-imaging-center-bic