contest_2019_LM Posted on October 23, 2019 by biu_0711 LM01 Neuron psychedelia microscope: Nikon spinning disk description: Time color-coded dynamics of actin and lysosomes in young hippocampal neurons Author: Mithila Burute LM02 Spirit and Tranquil microscope: Nikon Upright Microscope description: The human retinal pigment epithelium (hRPE) cells that treated with Centrinone, a compound that inhibit the mature of centriole, lost the ability to form microtubule organization center, thus leads to a failure to form the spindle and to dividing; and the cells changed to multi-nuclear cells. Red: microtubule, Cyan: nuclear Author: Fangrui Chen LM03 Hubrecht microscope: Confocal Leica SP8 description: Whole-mount staining of a pancreatic organoid. The organoid is shaped like an embryo (Hubrecht logo). Therefore I will simply call it “Hubrecht” Author: Tim Dielen LM04 Phoenix microscope: Olympus BX60 Fluorescence microscope description: TH and DsRed double staining in brain slices of rats treated with DREADD technology Author: Judith Hendriks LM05 Spiral of inhibitory neurons microscope: Confocal Zeiss LSM700 description: Here shown is an organotypic hippocampal slice of a mouse brain, which was kept in culture for over 2 weeks before fixation. Labelled in blue are all cell bodies (DAPI), to show the outer contours of the slice. In green is the genetic labelling of GAD65-GFP, indicating a subset of inhibitory neurons that express this protein Author: Lotte Herstel LM06 Knockin’ in memories microscope: Zeiss LSM700 description: CRISPR knock-in for PSD95, in the hippocampal formation of mouse brain Author: Arthur Jong LM07 Actin everywhere! microscope: Leica TCS SP8 STED 3X, 93x/1.30 glycerol objective description: STED image of actin in an human intestinal organoid resolving individual microvilli, depth encoded Z projection Author: Wilco Nijenhuis LM08 Night of the globular waves microscope: Leica DMI 6000 CS AFC (STED) description: Spreading of breast cancer cells on a 2D surface. The image shows the cytoskeletal fibers of the cells which are polymers of globular proteins. Microtubules (red), F-actin (cyan), nuclei (blue). Author: Milena Pasolli LM09 Neuronal sunflowers microscope: Confocal microscope LSM 880. description: Motor neurons were derived from human induced pluripotent stem cells and stained with neuronal markers Author: Svetlana Pasteuning-Vuhman LM10 Shooting Stars microscope: TIRF Nikon Ti – Roper iLas-2 FRAP/Photoablation system description: Image shows single frame of anti-parallel microtubule bundles reconstituted in vitro on a glass surface. The idea of this experiment was to create bundles of two anti-parallel microtubules by adding a microtubule bundling protein PRC1 (green). However, a little bit higher concentration of PRC1 produced bundles of multiple microtubules of similar size (depicted by red comets, marked by microtubule plus-end marker EB3) Author: Dipti Rai LM11 Hairy Pancreatic Cancer Cell microscope: ZEISS LSM700 description: F-actin staining of strongly adherent Pancreatic Ductal Adenocarcinoma cells Author: Joep Sprangers LM12 My heart beats for science microscope: Spinning Disc 1 Nikon-Roper ILAS PhotoAblation (Kruyt building) description: This is a compilation of pictures to form a heart shape. The pictures show a zoom of a human induced-pluripotent stem cell-derived cardiomyocyte, which was transduced with lentiviral vectors to express a GFP-tagged microtubule plus-end marker (end-binding protein 3 (EB3)) and tagRFP-LifeAct; an actin marker. In cardiac cells the actin cytoskeleton forms contractile units named sarcomeres, which allow the cell to beat Author: Babet van der Vaart LM14 Sun is a droplet microscope: Nikon upright Ni microscope description: Author: Chao Yang