Confocal Yokogawa Spinning Disc X1 – Nikon Ti – Roper iLas FRAP/Photoablation system

Confocal Yokogawa Spinning Disc X1 Nikon Ti Roper iLas FRAPPhotoablation system
Microscopy facility manager Ilya Grigoriev, in front of Spinning Disc microscope


Primary tasks:
Confocal Spinning Disc microscopy, 3D imaging, FRAP, Photo-Activation, Photo-Ablation (355 nm)

Microscope: Nikon Eclipse Ti with Perfect Focus System

Confocal Head: Yokogawa CSU-X1-A1

Apo TIRF 100x N.A. 1.49 oil, S Fluor 100x N.A. 0.5-1.3 (for 355 nm) oil, Plan Apo VC 100x N.A.
1.40 oil, Plan Apo VC 60x N.A. 1.40 oil, Plan Fluor 40x N.A. 1.30 oil, Plan Apo 20x N.A. 0.75,
Plan Apo 10x N.A. 0.45

Beamsplitter: DV2 (Two-channel, simultaneous-imaging system)

Camera: Photometrics Evolve 512 EMCCD

Vortran Stradus 405 nm (100 mW)
Cobolt Calypso 491 nm (100 mW)
Cobolt Jive 561 nm (100 mW)
Vortran Stradus 642 nm (110 mW)
Teem Photonics 355 nm Q-switched pulsed laser (for photoablation)

FRAP: ILas system (Roper Scientific France/ PICT-IBiSA, Institut Curie)

ET-DAPI (49000), ET-CFP (49001), ET-GFP (49002), ET-YFP (49003), ET-mCherry (49008),
ET-CFP/YFP (59017), ET-GFPmCherry (59022) – all Chroma

Stage: ASI motorized stage MS-2000-XYZ with Piezo Top Plate

Filter Wheels: Sutter LB10-3 (excitation filter wheel with smart shutter; smart shutter for
transmission light)

Heating: Tokai Hit INUBG2E-ZILCS Stage Top Incubator

Software: MetaMorph 7.7

Resolution: 67 nm in 1 pixel (100x objective)



Dr. Ilya Grigoriev
The head of the Biology Imaging Center
Division of Cell Biology, Department of Biology, Faculty of Science, Utrecht University

Room O-504
Padualaan 8, 3584 CH Utrecht,
The Netherlands