TIRF Nikon Ti – Roper iLas FRAP/Photoablation system

TIRF Nikon Ti Roper iLas FRAPPhotoablation system
Andrea Marques and Stine Morthorst are studying the exocytosis of Rab6 vesicles

Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)

Microscope: Nikon Eclipse Ti with Perfect Focus System

Objective: Nikon Apo TIRF 100x N.A. 1.49 oil

Beamsplitter: Optosplit III (allows two or three color simultaneous imaging)

Photometrics Evolve 512 EMCCD
Photometrics CoolSNAP HQ2 CCD

Vortran Stradus 405 nm (100 mW)
Cobolt Calypso 491 nm (100 mW)
Cobolt Jive 561 nm (100 mW)
Vortran Stradus 642 nm (110 mW)
Teem Photonics 532 nm Q-switched pulsed laser (for photoablation)

FRAP: ILas system (Roper Scientific France/ PICT-IBiSA, Institut Curie)

ET-DAPI (49000), ET-CFP (49001), ET-GFP (49002), ET-YFP (49003), ET-DSRed (49005), ETCy5
(49006), ET-mCherry (49008), ET-CFP/YFP (59017), ET-GFPmCherry (59022) – all Chroma

Stage: ASI motorized stage MS-2000-XY System for Inverted Microscope Nikon Te/Ti 2000

Filter Wheels: Sutter LB10-3 (excitation filter wheel with smart shutter; emission filter wheel; smart shutter for transmission light)

Heating: Tokai Hit INUBG2E-ZILCS Stage Top Incubator

Software: MetaMorph 7.7

Resolution: 63 nm in 1 pixel



Dr. Ilya Grigoriev
The head of the Biology Imaging Center
Division of Cell Biology, Department of Biology, Faculty of Science, Utrecht University

Room O-504
Padualaan 8, 3584 CH Utrecht,
The Netherlands