Dr. Katarina Wolf, RIMLS, Dept. of Cell Biology, Radboudumc Nijmegen
Nonlinear optical microscopy by multiphoton excitation has developed into a powerful approach that combines different excitation and emission techniques for the three-dimensional (3D) reconstruction of biological specimens over time (4D microscopy). The speciﬁc approaches include detection of two- and three-photon excited ﬂuorescence, two-photon excited ﬂuorescence lifetime (FLIM), and the second and third harmonic generation for the visualization of native tissue structures. Cancer progression imaging is performed in different models such as collagen lattices in vitro, mouse imaging window models in vivo and human skin models ex vivo. Using dynamic imaging examples of cancer invasion for mapping of single-cell and collective invasion mode, and the underlying guidance principles by tissue structures together with therapeutic interventions, the power of multiphoton microscopy will be demonstrated.